Bibliography
…ELECTROCHEMISTRY, Vol.17 No.1, Feb 2011 Ellyn Kerr, “Surface Plasmon Resonance Branches Out : Workhorse Label-Free Drug Discovery and Development Tool Tackles New Applications”, Genetic Engineering & Biotechnology News. February 15,…
Application Note 141: SPRM Measurements of Binding Kinetics between Rituximab and CD20 on Live B Cells
…designed for studies of live cells, such as FACS and LigandTracer, SPRM is a label-free technique for measuring binding kinetics and affinity in real time. The system integrates SPR and…
Biosensing Instrument is a Fall 2019 Arizona Innovation Challenge awardee
…at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA) is the state’s leading economic development organization with a streamlined mission to grow and strengthen Arizona’s economy. The ACA…
Biosensing Instrument named “Best Specialty Life Science Instruments Supplier” in Global Health & Pharma’s 2020 Biotechnology Awards
…our full winner listing, please visit the GHP website. GHP’s official press release can be accessed at https://www.ghp-news.com/ghp-news-2020-biotechnology-awards-winners-press-release/ About Biosensing Instrument Biosensing Instrument, Inc. (BI) is a privately held company based…
Application Note: 113 – Flow-Through Electrochemical Surface Plasmon Resonance (SPR): Detection of Intermediate Reaction Products
…along the flow path, which opens the door to many new applications. For example, one can generate an electrochemical reaction at an upstream location and detect the reaction products at…
Biosensing Instrument
Measurement Services Available Try out our real-time, label-free, SPR binding assay on whole cells in their native environment. Learn More COVID-19 research with SPRM New cell-based kinetic binding studies with…
Application Note 126: GPCR Binding Assays with SPR Microscopy
…Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 126: GPCR Binding Assays with SPR Microscopy Download Now Note References Locatelli-Hoops, S et al,…
We are hiring!
Come and join us to be part of an exciting company designing cutting edge technology and award winning products for the early drug discovery market! We are looking for a Technical…
Biosensing Instrument exhibiting at the 2019 Antibody Engineering & Therapeutics conference
…will be exhibited at the conference. SPRm 200 is the world’s first commercially available surface plasmon resonance microscopy (SPRM) system and it is the only scientific solution on the market…
Why Surface Plasmon Resonance?
…i.e. ELISA, QCM, and ITC. However, few encompass as many advantages as Surface Plasmon Resonance. SPR enables (1) high sensitivity, (2) label-free detection, (3) real-time monitoring, (4) low volume sample…
Free webinar on Jan 28 2021: SPR for Biosensor Applications
…combined with other techniques such as electrochemistry or gas, it allows one to obtain new insight into metal-solution or membrane-gas interfacial processes, enabling new applications and technology development in biosensor…
Application Note: 108 – SPR as a Chromatographic Detector: Separation and Label-Free Detection of Biomolecules
…innovative research and novel applications. For example, one channel of the dual SPR cell can be used for detection of chromatographic eluents, while the other channel, through which only the…
Biosensing Instrument is a Semifinalist in the Fall 2019 Arizona Innovation Challenge
…of panels and workshops within the subsequent 12 months of the award. Media Contact Connie Weber, Arizona Commerce Authority, conniew@azcommerce.com About the Arizona Commerce Authority The Arizona Commerce Authority (ACA)…
Environment, Food & Beyond
…step forward in monitoring environmental safety. Development of new SPR techniques The versatility of BI’s SPR instruments open up endless possibilities for research. Our high-sensitivity and flexible designs allow for…
Application Note 123: Surface Plasmon Resonance Measurement of Protein-Peptide Interaction Using Streptavidin Sensor Chip
…Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 123: Surface Plasmon Resonance Measurement of Protein-Peptide Interaction Using Streptavidin Sensor Chip Download Now Note…
Application Note 117: Real-time Monitoring Biomarker Expression of Carcinoma Cells
…and antineoplastic drug development. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 117: Real-time Monitoring Biomarker Expression of Carcinoma…
Application Note 140: Membrane Transport Protein Binding Kinetics Using Label-Free SPRm200
…obtain using traditional labelled and label-free methods. Measuring biophysical properties in the membrane target’s native environment in real time and label free provides insightful drug-receptor interactions information much earlier in…
Technical Note 103: Surface Plasmon Resonance v. Quartz Crystal Microbalance
Surface Plasmon Resonance v. Quartz Crystal Microbalance Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM) are two powerful label-free detection techniques that are receiving increasing amounts of attention for…
Solutions
SPR Microscopy This award winning technology opens a new frontier in the label-free molecular interactions that combines SPR with optical microscopy. Designed specifically to measure binding affinity and kinetics of…
Application Note 122: Surface Plasmon Resonance Microscopy for Multiple and Single Cell Membrane Binding Kinetics Studies
Surface Plasmon Resonance (SPR) is a powerful technique for measuring the binding kinetics of biomolecular interactions in a real-time and label-free manner [1]. In traditional SPR assays, the target…
Surface Plasmon Resonance
…such molecular binding studies, i.e. ELISA, QCM, and ITC. However, few encompass as many advantages as Surface Plasmon Resonance. SPR enables (1) high sensitivity, (2) label-free detection, (3) real-time monitoring,…
Application Note 157: Understanding the Glyco-code using SPR microscopy
…heterogeneity in real-time in whole cells. Furthermore, profiling the cell surface glycosylation using lectins not only plays a critical role in understanding the function of the complex glycocalyx in tumorigenesis,…
Application Note 131: SPR Microscopy for Live Suspension Cells
SPRm 200 system is the world’s first commercial Surface Plasmon Resonance Microscopy (SPRM) which combines the high spatial resolution of optical microscopy with the label-free sensing capability of SPR. With…
Application Note: 110 – Studying Protein Adsorption Properties with SPR
…We would like to thank Dr. Chae for sharing his results with us. DOWNLOAD PDF Download a PDF of Application Note: 110 – Studying Protein Adsorption Properties with SPR Download…
Application Note 102: Electrochemical SPR: Redox-Induced Protein Conformational Changes
Both electrochemical and surface plasmon resonance (SPR) techniques measure various processes taking place at or near an electrode surface. Combining the two techniques allows one to obtain new insight into…
Application Note: 107 – Binding Kinetics Analysis with SPR: Interaction between Bovine Serum Albumin (BSA) and Anti-BSA
…the binding interaction of the anti-BSA/BSA immune complex in real-time. The BI Autosampler can run two well-plates in any combination of 12, 96, and 384 well formats for a maximum…
Application Note 146: ENHANCED REDUCTION OF NONSPECIFIC INTERACTIONS
…region in which cells were permitted to grow freely. The red square indicates an example area for detection that includes both cell regions and FBS-free regions for referencing. III. Data…
Application Note 132: SR142948 Antagonist Binding to NTS-1 Receptor Using SPR Microscopy
…Dr. Scott Pollack from Sygnature Discovery for the samples, data and valuable insights. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of…
Application Note: 105 – Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin
…0.4, 1.0 µM) were injected. A flow rate of 80 µL/min was used. Author: Nguyen Ly | Biosensing Instrument | Published July 1, 2012 DOWNLOAD PDF Download a PDF of…
Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
…-1), off-rate (kd = 1.16E-03 s -1) and binding affinity (KD = 5.49 nM) were reported. Importantly, the mean KD of chemerin binding to huCCRL2 determined by SPRM was comparable…
Arizona Commerce Authority Announces the Fall 2019 Arizona Innovation Challenge Finalists
…Application areas include life sciences, drug discovery, antibody characterization, biosensors and electrochemistry. Information about BI can be found at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA)…
Life Science
Biosensing Instrument makes instruments to perform binding kinetic assays on purified proteins and whole cells in real time, in vitro, and label-free. For cell-based binding assays, the SPRm 200 platform…
Application Note: 111 – Measuring Surface Charge Density: A New Application with SPR
…are caused by surface regeneration. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note: 111 – Measuring Surface Charge Density:…
Application Note 159: Real-Time Monitoring of Glycan Heterogeneity in Cancer Cells
…O-linked glycosylation is critical for the discovery of new diagnostics and cancer biomarkers, and to understand the function of tumorigenic membrane glycoproteins within the complex glycosylated cancer cellular environment. Lectins…
Biosensing Instrument graduates the Venture Ready Accelerator program
…electrochemistry. Information about BI can be found at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA) is the state’s leading economic development organization with a streamlined mission…
Chemical Vapor Sensing
…detectors that analyze chemical components in vapors such as human breath. Using these SPR methods could have a huge significance for a variety of health-related studies and medical research overall….
EC-DualFlow™ Analysis Module
…valuable biological samples. The dual-channel design allows users to perform serial downstream analysis, control experiments, and develop new applications. Key Features Performance Characteristics Dual-channel electrochemical SPR with flow control BI-DirectFlow™…
Application Note 155: Rapid Determination of IgG in Sera using BI-4500A SPR System
…University Hospital. As shown in Table1, the relative differences between the RD SPR method results and those obtained using a commercial immunoturbidimetric kit are all less than 10%. Moreover, a…
Application Note 151: Studying Cell Binding Heterogeneity using SPRm 200
…numerous physiological and pathological processes leading to new strategies in drug design and discovery2. Cell heterogeneity is always present to some level in most cell populations. Also, cell-to-cell differences can…
Application Note 133: Interaction of Protein Phosphatase 1 with its Muscle Glycogen– targeting Regulatory Subunit Measured by SPR
…was measured with the help from Biosensing Instruments researchers. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 133: Interaction…
Bi-DirectFlow™ Analysis Module
…mass limited transport, and non-specific binding. As a result, high quality data that is more representative of true molecular binding behavior is obtained. Multiple Channels, Small internal volume The dual…
Gas Phase SPR
…metal film with sensing materials such as polymers (Figure 1), adsorption of the molecules onto the sensing materials can be detected by observing SPR angle shift. FIG. 1 Chemical vapor…
EC-SPR Analysis Module
…SPR instruments for high performance EC-SPR analysis. Bio and electrochemical compatible The module’s body and fluidic components are constructed with biocompatible materials. Such biocompatibility eliminates sample carryover and memory effects….
Flow Injection Analysis Module
…valuable samples. Biocompatibility The cell body and fluidic channels are constructed with biocompatible materials. Such biocompatibility eliminates sample carryovers and memory effects, affording continuous and undisrupted sample injections and analyses….
FCOI Policy
…applications for Phase I support under the Small Business Innovation Research (SBIR) and Small Business Technology Transfer (STTR) programs. Compliance and Penalties for Non-Performance If an investigator fails to comply…
Application Note 120: Electrochemical SPR Monitoring of Drug Interactions with Live Cancer Cells
Surface Plasmon Resonance (SPR) has emerged as a sensitive, real-time and label free technique for quantifying biomolecular interactions.[1] By combining SPR with electrochemical methods, many new capabilities and applications are now…
SPRm 200 Series
…measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR. Click here to download a PDF datasheet. Key Features System Specifications References Integrated…
Free webinar on May 25 2021: Applications of EC-SPR and SPRm Impedance
Biosensing Instrument will be hosting a free webinar in collaboration with California State University, Los Angeles and Admiral Instruments on May 25 2021, 9am LA / noon NY / 5pm…
Biosensing Instrument attending 9th NovAliX Conference
…will present at #novalixconference on Kinetic Characterization of ligand and antibody binding to cell surface expressed CCRL2 using #SPRm200 at IL01 1:10pm on 4/26/2023. https://www.novalix-conferences.org/programme For more information about the meeting, go to https://www.novalix-conferences.org…
BIOSENSING INSTRUMENT EXHIBITING AT THE 2022 Drug Discovery Chemistry Conference
…unique scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.drugdiscoverychemistry.com/…
BIOSENSING INSTRUMENT EXHIBITING AT Drug Discovery Chemistry 2023
…unique scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the meeting, go to https://www.drugdiscoverychemistry.com/programs…
Biosensing Instrument exhibiting at the 19th Discovery on Target conference
…the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.discoveryontarget.com/…
Biosensing Instrument speaking and exhibiting at the 2020 Discovery on Target Virtual conference
…only scientific solution in the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins directly on whole cells. For more information about the conference: https://www.discoveryontarget.com/…
Application Note 130: Measuring molecular binding kinetics with SPRM Impedance
…to new applications. A recent example is the integration of SPRM with electrochemical methods for molecular binding kinetics quantification. Researchers at Arizona State University and Johns Hopkins University developed a…
Application Note 128: SPR Microscopy for Acid-Sensing Ion Channels
…| Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 128: SPR Microscopy for Acid-Sensing Ion Channels Download Now Note References Lingueglia, E et…
Application Note 127: Quantifying Antibody Binding to Membrane Proteins on Single Cells with SPR Microscopy
…| Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 127: Quantifying Antibody Binding to Membrane Proteins on Single Cells with SPR Microscopy Download Now Note References…
Application Note 125: Lectin-Glycoprotein Interactions with SPR Microscopy
…Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 125: Lectin-Glycoprotein Interactions with SPR Microscopy Download Now Note References Patching, S.,…
Application Note 114: SPR Binding Affinity Determination of Novel Peptide Inhibitors to the Innate Immune activator C1q
…to remove immune complexes and apoptotic cells. Three different pathways comprise the complement system: the classical, lectin and alternative pathways [1]. While the complement system plays a central role in…
BIOSENSING INSTRUMENT EXHIBITING AT DISCOVERY ON TARGET 2024
…exhibit at Discovery on Target 2024 on October 1 – 3, 2024 in Boston, Massachusetts. Please visit us at booth 421, For more information about this conference, go to https://www.discoveryontarget.com/…
Contact Us
…1007 E Warner Rd #102 Tempe, AZ 85284 +1 480-491-2777 We have a large distribution network around the world! Contact us for more information. CONTACT SALES info2BI@Biosensinginstrument.com CONTACT SUPPORT support@Biosensinginstrument.com…
BIOSENSING INSTRUMENT EXHIBITING AT Drug DISCOVERY Chemistry 2024
…will exhibit at Drug Discovery Chemistry 2024 on April 1st-4th, 2024 in San Diego, California. Please visit us at booth 511, For more information about this conference, go to https://www.drugdiscoverychemistry.com…
Application Note 103: Electrochemical SPR: Metal Deposition/Stripping and Reorganization of Electroactive Organic Thin Films
…Ly | Biosensing Instrument | Published May 1, 2010 DOWNLOAD PDF Download a PDF of Application Note 103: Electrochemical SPR: Metal Deposition/Stripping and Reorganization of Electroactive Organic Thin Films Download…
Electrochemical Measurements
…gold film and monitored in real-time. Electrochemical research has continued to expand with the development of new polymer surfaces, which can easily be tested by using our interchangeable analysis modules….
Application Note 124: Quantifying Molecular binding to Membrane Proteins on Individual Cells with Surface Plasmon Resonance Microscopy
SPRm 200 System is the world’s first commercial Surface Plasmon Resonance Microscopy (SPRM). It combines the high spatial resolution of optical microscopy with the powerful sensing capability of SPR, making…
Application Note 119: Small Molecule Detection by Surface Plasmon Resonance (SPR)
…disorders, and discover antifungal and antibacterial agents. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 119: Small Molecule Detection…
Application Note: 109 – Application of SPR to Bacteriology: Endotoxin/Protein Interaction Studies
…for his technical help, discussion, and the provision of the CD-14 protein and endotoxin samples. DOWNLOAD PDF Download a PDF of Application Note: 109 – Application of SPR to Bacteriology:…
Application Note 106: Flow Injection-SPR: Sensitive and Sequence-Specific DNA Assays
…amplification scheme. Author: Nguyen Ly | Biosensing Instrument | Published June 11, 2014 DOWNLOAD PDF Download a PDF of Application Note 106: Flow Injection-SPR: Sensitive and Sequence-Specific DNA Assays Download…
Application Note 104: Flow-Injection SPR: Sensitive Determinations of Heavy Metal Ions
…2010 DOWNLOAD PDF Download a PDF of Application Note 104: Flow-Injection SPR: Sensitive Determinations of Heavy Metal Ions Download Now Note References Chah, S.; Yi, J.; Zare, R. N. Sens….
Technical Note 106: SPR Microscopy vs Radioligand Binding Analysis
…(ka) and dissociation (kd) kinetics. Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for measuring both the equilibrium dissociation constant, and the kinetic constants. Since its commercialization…
Application Note 147: Variations in the Binding Kinetics of Native and Recombinant Protein Receptors
…to HER2 in SKBR3 cells was studied with a SPRm200 system14 and compared to the kinetic binding values obtained from isolated rHER2. The SKBR3 cells were incubated and allowed to…
Application Note 136: Sensitivity and kinetic analysis of carboxyl-graphene oxide-based SPR biosensors for label-free detection of lung cancer biomarker
…a modified Hummer method, yielding a carboxylate product from chloroacetic acid (Cl–CH2–COOH) under basic conditions. A carboxyl-GO sheet has easily synthesized functional groups, which form unique composites (2). Using an assay with…
ON DEMAND CELL-BASED SPRm WEBINAR WITH Dr. Rives
…with a variety of small molecule and antibody binding examples. Topics from this webinar: Overview of SPRm principle Review case studies of small molecule pH-dependent binding of GPR4 Explore label-free…
Cell-based Measurement
…time. Have flexibility to meet your unique requirements. Benefit Process Cell-based assay with SPRm 200 Real-time, in-vitro, label-free kinetic measurements with simultaneous optical imaging for quantitative binding activity mapping on…
Application Note 129: AZ1395 small molecule targeting GPR39
…Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 129: AZ1395 small molecule targeting GPR39 Download Now Note References Tang,…
Application Note 115: SPR Assay of Clinical Alzheimer Disease Samples: Amyloid β Peptides in Cerebrospinal Fluids
…Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 115: SPR Assay of Clinical Alzheimer Disease Samples: Amyloid β Peptides in Cerebrospinal Fluids Download Now Note References…
Application Note 101: Fast Kinetics of Conformational Changes in Proteins
…be with simple exponential functions. However, excellent fits were obtained by using a stretched exponential function, exp[-(t/t)b], with b~ 0.5. Stretched exponential processes are quite common in complex systems, such…
Application Note 121: Protein Interactions in the Mycofactocin Biosynthetic Pathway
…KD (C). Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 121: Protein Interactions in the Mycofactocin Biosynthetic Pathway Download…
Application Note 118: SPR for Food Safety and Monitoring: Melamine in Milk Samples
…performed for simple, reliable, and fast testing of melamine in milk. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note…
Application Note: 112 – Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell Lysates
…Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note: 112 – Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell…
Application Note 116: Temperature Dependence of Enzymatic Cleaving Activity
…an important feature for SPR measurements. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 116: Temperature Dependence of Enzymatic…
Application Note 160: Influence of Aberrant Glycosylation on the Binding Capability of Muc-4 in Pancreatic Cancer Cells
…complex influences of the native N-glycan cellular environment on binding interactions to the Muc-4 receptor as this is currently the only commercially available label-free technique with high enough sensitivity and…
Webinar with Pfizer: Kinetic characterization of ligand & antibody binding to cell surface expressed CCRL2
…Plasmon Resonance Microscopy) is a powerful technology that enables label-free kinetic binding interaction studies of drug molecules with whole cells in real-time. By combining traditional cell culture and imaging techniques…
Biosensing Instrument Exhibiting at the 2021 Antibody Engineering and Therapeutics
…the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://informaconnect.com/antibody-engineering-therapeutics/…
BIOSENSING INSTRUMENT EXHIBITING AT ACS Spring 2023
…label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. Please visit us at booth 509. For more information about the meeting, go to https://www.acs.org/meetings/acs-meetings/spring-2023.html…
BIOSENSING INSTRUMENT ATTENDING BPS2023
…that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment, Please visit us at Booth 919. For more information, go to https://www.biophysics.org/2023meeting#/…
Application Note 143: Monitoring Electrochemical Activities of Single Prussian Blue Nanoparticles with SPRm Electrochemistry
Prussian blue is a pigment that is used to color paints, inks, textiles, and other commercial product (1). Prussian blue nanoparticles (PBNPs) are nanomaterials that present unique properties and excellent…
Biosensing Instrument presenting at the 2nd NovAliX Virtual Conference
…solution on the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.novalix-conferences.org/…
Technical Note 105: Introduction of SPR Microscopy
Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for studying binding behavior of biomolecules. Since its commercialization in 1990’s [1], SPR has made vast advances in terms…
BI-4500 Series
…for advanced five-channel ultra-fast kinetics research Optional Accessories: EC-DualFlow™ Module for advanced two-channel electrochemical (EC) flow SPR research EC-SPR Module for electrochemical (EC) SPR research Gas SPR Module for gas…
BI-2500 Series
Ideal system for developing biosensors in your research. Its flexible design allows an upgrade path for electrochemical SPR or chemical vapor (gas) SPR combination studies. Click here to download a…
SPRm200 webinar with Genentech: Exploration of avidities between monovalent and bivalent antibodies in correlation with cellular expression
…Microscopy) is a novel technology that enables label-free and real-time binding affinity and kinetics of membrane proteins with drug molecules. By combining cell culture and imaging techniques with biophysical assays,…
Biosensing Instrument exhibiting at CHI’s 18th Annual PepTalk
…affinity and kinetics of cell membrane proteins in their native environment at the single cell level. Biosensing Instrument will be at booth #423. For more information about the conference: www.chi-peptalk.com…
How Does Surface Plasmon Resonance Work?
…oscillations of free electrons (known as surface plasmons) via the Kretschmann configuration, in which light is focused onto a metal film through a glass prism and the subsequent reflection is…
Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
…with increased acidity, Compound 13 is unlikely the appropriate compound to study GPR4 and its role in inflammatory bowel conditions. Compound 13 and its analogues’ pH dependence strongly limits their…
Webinar Registration
…RepublicLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacaoMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorth MacedoniaNorthern Mariana IslandsNorwayOmanPakistanPalauPalestine, State ofPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarRomaniaRussian FederationRwandaRéunionSaint BarthélemySaint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent…
Application Note 153: Differentiating Mono and Bivalent Antibody Binding in MCF7 Cell line
Understanding antibody binding and kinetics of therapeutic antibodies to tumor cells plays a critical role in advancing therapeutics. SPR is commonly used to study antibody binding reactions. However, it has…
Application Note 137: Ginnalin A and Tabersonine Inhibition of Amyloid β(1-42) Aggregation
…on neurons (3). In this study, the binding interactions of two natural compounds with Αβ(1-42) were measured. Ginnalin A (GA), a phenolic compound isolated from the red maple (Acer rubrum)…
BIOSENSING INSTRUMENT EXHIBITING AT DISCOVERY ON TARGET 2023
…on #SPRm200, and our research on “Binding interactions to key potential pancreatic cancer vaccine candidate (Mucin-4) are significantly hindered by aberrant glycosylation of pancreatic cancer cell”. For more information about…
End of Support Reminder for BI-2000, BI-3000 and BI-4000 SPR systems
…part orders until December 31, 2020. We are offering trade-in incentives to upgrade to the award winning 5 channel SPR (BI-4500) or the 3 channel SPR (BI-2500). Contact info2bi@biosensingusa.com for more info….
Accessories
SENSOR CHIPS We provide high-quality sensor chips to address a variety of research needs. BARE GOLD SENSOR CHIP High film uniformity for reproducible SPR research. DIVIDED GOLD SENSOR CHIP Pre-patterned…
Application Note 134: Positive and Negative Control Studies of Small Molecule Binding to Membrane Protein
…SPR and optical microscopy into one platform, which resolves individual cells spatially and quantity ligand binding to each of them in real time and without labels. In this application note,…
Resources
We encourage you to explore our technical documentation below to learn more about Surface Plasmon Resonance Technology and how it can be applied to your research. APPLICATION NOTES These short…
Technologies
SURFACE PLASMON RESONANCE Surface Plasmon Resonance (SPR) has emerged as a powerful detection technique due to its high sensitivity and label-free capability. This optical-based real-time detection method delivers superior results…
SPR Microscopy
…Real-time interactions of the drug and membrane protein can be measured in its native state without needing to extract proteins from the cell. Because of its unique measurement of cell…
Technical Notes
…Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for studying binding behavior of biomolecules. Since its commercialization in 1990’s [1], SPR has made vast advances in terms…
Application Note 154: Measuring Affinity and Avidity under High and Low Expression Conditions
…of antibodies are the characteristics which define the overall efficacy of binding kinetics.1 These properties enable a new wave of antibody drugs with tailored properties, promising improved therapeutics.2 Hence, a…
Application Note 142: Small Molecule Binding to Membrane Transporter Using SPRm200
…free manner. However, measuring membrane proteins binding with the SPR has been a challenge because of the need to immobilize purified receptors on the sensor chip surface. SPR microscopy (SPRm)…
Application Note 149: The Effect of Cell Fixation on Binding Interaction Kinetics
…However, this approach can be too harsh, causing dehydration and precipitation of cellular components, and possibly even reducing the activity of certain membrane proteins. Figure 1: Schematic of the impacts…
On Demand SPRm200 Webinar with Pfizer
Hosted by Labroots, a free SPRm200 webinar with Biosensing Instrument and Pfizer is now available online. In this webinar, Pfizer will show how they utilized SPRM along with other biophysical…
Analysis Modules
…Click here to download a PDF datasheet. FLOW INJECTION SPR Flow injection SPR allows SPR analysis to be carried out in a continuous liquid stream. Read More ELECTROCHEMICAL SPR Electrochemical…
Technical Note 107: Basic Principles of SPRm Impedance
…imaging local electrochemical impedance in real time with high spatial resolution. This technique relies on detecting surface plasmon waves propagating on a metal thin film which are created by a…
Technical Note 104: Concepts Behind EC-SPR
Voltametric technique in electrochemical (EC) detection is a very powerful analytical method that has been used for a wide range of applications. When combined with SPR technology, EC-SPR provides many…
Application Note 144: Single Cell Apoptosis Monitoring with SPRm Impedance
…commonly used in material science for corrosion studies but its use in biological applications have grown rapidly and this technique is now used to monitor cell adhesion, growth, differentiation and…
Application Note 145: KINETIC MEASUREMENTS OF COVID-19 VIRAL S1 PROTEIN WITH CELLULAR AND RECOMBINANT ACE2 RECEPTORS
…relevant for drug development and testing. Surface plasmon resonance microscopy (SPRM), such as BI’s SPRm200, integrates label-free, high-resolution SPR binding kinetic measurements with bright-field optical microscopy for the study of…
BIOSENSING INSTRUMENT ATTENDING THE 2022 DISCOVERY ON TARGET CONFERENCE
…scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment along with a talk by Jonathan Brooks from Pfizer ”…
Application Note 139: Single-Injection Kinetic Measurement Technique for Studies of COVID-19 S1 Protein and ACE2 Binding
…the ligand before a new analysis. This is accomplished by introducing a reagent such as acid, base, surfactant, or other compounds to dissociate the preformed bioconjugates at the surface. However,…
Modular
To meet the growing needs of the research community and to assist innovative users in developing new applications, BI SPR uses a patented creative design that offers flexibility and versatility…
Small Molecule Detection
…component for the high performance of any SPR instrument. Our instrument utilizes an enhanced position-sensitive device with advanced algorithm to detect extremely small SPR angle shift. Combining with the noise…
Gas SPR Analysis Module
Biosensing Instrument Inc. introduces the first commercially available Gas SPR detection system. This technology enables the high sensitivity of SPR analysis to be performed in the gas phase, permitting new…
Technical Note 102: SPR Sensitivity and Detection Limit
…of sensitivity between units of degree angular shift and RIU requires careful consideration of instrumental conditions. Example 5 How do the following SPR instruments compare? The first instrument has a BK7…
BI SPR Technology
At Biosensing Instrument Inc., we use a unique detection method to achieve both high sensitivity and fast detection speeds. Our innovative design and BI-DirectFlow™ technology allows us to combine precise…
Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
…(a) Bright-field image and (b) corresponding SPR image of the SKBR3 cells with red squares representing areas of binding activity (ROIs). C) A collection of example sensorgrams obtained from responsive…