The cell-based Surface Plasmon Resonance Microscopy (SPRM) system, integrates optical microscopy and SPR, is a powerful technique for measuring binding activities of membrane proteins in vitro. It allows the simultaneous measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR.
Click here to download a PDF datasheet.
- Integrated optical microscopy with SPR Bright-field and SPR microscopies in one instrument. Large field of view with high resolution optics to view single or multiple cells.
- In vitro and label-free binding activities mapping Provides SPR sensorgrams and binding activity map of individual cells as well as the binding kinetic constants (ka, kd, KD).
- Nanometer scaled binding response of virus, bacteria and nanoparticles Nanomotion detection study of bacteria or virus metabolic activity or binding activities of nanoparticles for drug delivery designs.
- Technical note #105 “Introduction of SPR Microscopy” TechNote105
- Technical note #106 “SPR Microscopy vs Radioligand Binding Analysis” TechNote106
- Application Note 129: AZ1395 small molecule targeting GPR39
- Application Note 132: SR142948 Antagonist Binding to NTS-1 Receptor Using SPR Microscopy
- Application Note 141: SPRM Measurements of Binding Kinetics between Rituximab and CD20 on Live B Cells
- Application Note 142: Small Molecule Binding to Membrane Transporter Using SPRm200
- Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
- Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
- Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
- Application Note 160: Influence of Aberrant Glycosylation on the Binding Capability of Muc-4 in Pancreatic Cancer Cells
- Application Note 165: Spatially Resolved Detection of Electroactive Biological Systems Using EC-SPR Microscopy
- Many other app notes: AppNotes page
- K Syal, R Iriya, Y Yang, H Yu, S Wang, S Haydel, HY Chen, NJ Tao, “Antimicrobial Susceptibility Test with Plasmonic Imaging and Tracking of Single Bacterial Motions on Nanometer Scale“, ACS Nano, 10, 845-852, 2016
- F Zhang, S Wang, L Yin, Y Yang, Y Guan, W Wang, H Xu, N.J. Tao, “Quantification of Epidermal Growth Factor Receptor Expression Level and Binding Kinetics on Cell Surfaces by Surface Plasmon Resonance Imaging“, Analytical Chemistry, 87(19), 9960-9965, 2015
- L Yin, W Wang, S Wang, F Zhang, S Zhang, N.J. Tao, “Measuring Binding Kinetics of Antibody-Conjugated Gold Nanoparticles with Intact Cells”, Small, 2015
- W Wang, Y Yang, S Wang, V Nagaraj, Q Liu, J Wu and N.J. Tao, “Label-free measuring and mapping of binding kinetics of membrane proteins in single living cells“, Nature Chemistry, 4, 846-853, 2012