Biosensing Instrument makes instruments to perform binding kinetic assays on purified proteins and whole cells in real time, in vitro, and label-free. For cell-based binding assays, the SPRm 200 platform measures drug compound interaction with membrane proteins/receptors in their native state directly, providing binding kinetics mapped onto each cell and enabling heterogeneity and avidity analysis. Read more in the SPR microscopy section.

For measuring biomolecular interactions, BI’s unique Flow Injection SPR technique (BI-DirectFlowTM) delivers sample compounds with near-zero dispersion to effectively remove spurious secondary effects in measurement. This enables fast and precise kinetics for high-quality results.


Check out our flow injection analysis modules for your life science research:

BI-DirectFlow™ analysis module
included in BI-4500 series

Flow Injection analysis module
included with BI-2500 series

Our flow injection SPR technique and high-sensitivity instruments are perfect for a variety of life science applications:

DNA-protein, protein-protein, and protein-drug interactions
Here at BI, our SPR technology generates real-time binding data that is useful in understanding the dynamic interactions between proteins and other biomolecules that drive everyday biological processes. By immobilizing one molecule onto a sensor chip and by carrying the other via buffer solution in a flow cell, our unique SPR techniques can provide important information about protein binding properties, bacteriology-related protein studies, protein mutation and affinity studies, proteomics, and much more. This, in turn, will elucidate protein functionality and many disease mechanisms, opening the door for even more life science applications.

Gene assays (e.g., single nucleotide polymorphisms) and DNA-DNA interaction
BI-SPR technology has proven to be powerful and effective for rapid, label-free genetic and DNA-DNA interaction analyses. By coating a sensor surface with a DNA probe, it can be used for affinity binding studies, concentration detection of target DNA, DNA/gene mutation studies, DNA hybridization detection studies, and more. Our unique BI-DirectFlow™ system ensures precision sample delivery with near-zero dispersion, which allows for higher-quality studies of fast kinetics and high-affinity binding processes than ever before.

Immunosensing (e.g., ligand-receptor, antibody-antigen, etc.)
Our BI-SPR techniques are very useful for label-free, real-time monitoring of the affinity and kinetics of antibody-antigen and ligand-receptor interactions. The concentration and distribution of target sites can yield important information about the functional consequences of binding, antibody characterization, immunogenicity, and more. Within research, antibodies have been used to elucidate details of biological processes and pinpoint therapeutic targets. Immunosensing is a powerful analytical technique that can be used for specific analysis and detection of a large variety of biomolecules, and our instruments provide the sensitivity necessary for superior results.

Conformational changes and unfolding in proteins
Conformational changes in proteins and other biomolecules are often small and very fast, making it difficult to measure and monitor. BI’s SPR techniques are capable of detecting these small conformational changes in surface-bound molecules, which would provide important information about the critical roles of proteins in many biological functions and processes. Our powerful and high-precision detection technology delivers superior, high-quality data even for the fastest molecular processes.

Drug discovery and development
Our BI-SPR technology can provide high-quality data on kinetics, affinity, and specificity– all useful interaction characteristics in the drug discovery and development process. Because of their high sensitivity, small sample usage, and convenient label-free technique, our instruments have become increasingly powerful tools that provide information about various drug compounds and drug affinity. Protein and antibody-interaction studies are also used to investigate disease pathways and targets for potential drug therapies, and much more. Here at BI, our high-sensitivity instruments provide superior results for small-molecule detection, ensuring the best results possible for your drug development research.