Cell-based SPR Microscopy Application in Drug Discovery
Labroots webinar (September 12 2023)
Speakers:
Dr. Marie-Laure Rives, Head of Biology, PostEra
Dr. Shijie Wu, Application Scientist, Biosensing Instrument
Key learnings:
– Overview of SPRm principle
– Review case studies of small molecule pH-dependent binding of GPR4
– Explore label-free GPCR binding affinity and kinetic measurement on whole cells
Cell-based SPR Microscopy with Antibodies Targeting GPCRs: applications with Pfizer
Labroots webinar (April 26 2022)
Speakers:
Dr. Shijie Wu, Application Scientist, Biosensing Instrument
Jonathan Brooks, Principal Scientific, Pfizer
Key learnings:
– Overview of SPRm principle
– Review case studies of small molecule and antibody binding
– Explore label-free GPCR binding affinity and kinetic measurement on whole cells
Label-free Binding Affinity and Kinetics of Cell Membrane Proteins
Discovery on Target 2021 conference talk (September 28 2021)
Speakers:
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learnings:
– Learn principles of SPR microscopy.
– Application examples of small molecule or antibody binding to cell membrane targets using SPRm200.
Análisis cuantitativos de interacciones biomoleculares libres de marcaje fluorescente basado en el fenómeno de resonancia de plasmones superficiales
Congreso Internacional de Biotecnología y Bioingeniería (Agosto 5 2021)
Disertador:
Ing. Akemi Ueki, VP Mercadeo y Ventas, Biosensing Instrument
Key learnings:
– Teoría básica de SPR y SPRm.
– Aplicaciones de SPR y SPRm200.
Single cell & label free binding affinity: applications with Genentech
Labroots webinar (June 15 2021)
Speakers:
Dr. Shijie Wu, Application Scientist, Biosensing Instrument
Elaine Mai, Senior Scientific Researcher, Genentech
Key learnings:
– Describe principles of SPRm.
– Review case studies of small molecule and antibody binding.
– Differentiate avidities between monovalent and bivalent antibodies in correlation with cellular expression.
Applications of Electrochemistry combined with SPR and SPRm
BI sponsored webinar (May 25 2021)
Speakers:
Dr. Sujan Shrestha, Applications Engineer, Admiral Instruments
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Prof. Yixian Wang, California State University, Los Angeles
Key learnings:
– Introduce basic principles of EC.
– Review fundamentals of SPR.
– Dive into electrochemical characterization of electrodeposited thin films and nanoparticle surface structural dynamics applications.
Introduction to SPRm200: a Cell-based SPR
2nd Virtual NovAliX Conference conference talk (March 12 2021)
Speaker:
Akemi Ueki, VP Business Development, Biosensing Instrument
Key learnings:
– 10min introduction of SPRm 200 and its applications.
Essentials to Design a SPR Experiment
Speaker:
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learning:
– Understand key elements needed to design a successful SPR experiment.
Electrochemistry-SPR & Gas-SPR for Biosensor Applications
Surface Ventures webinar (January 28 2021)
Speakers:
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Dr. Rand Ismaeel, Research Fellow, University of Southampton
Key learnings:
– Introduce basic principles of SPR.
– Review Electrochemistry-SPR applications.
– Show applications of gas-SPR technique by using methane permeable membranes.
Surface Plasmon Resonance Microscopy: Direct Measurement of Molecular Interactions of Membrane Proteins on Cell Surface
Discovery on Target 2020 conference talk (September 16 2020)
Speakers:
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learnings:
– Learn principles of SPR microscopy.
– Understand key differences with traditional SPR techniques.
– Application examples of GPCR (G-protein coupled receptors ) binding measurement with small molecules and antibodies.
Applications of Cell-based SPR Microscopy with AstraZeneca
Drug Target Review webinar (June 30 2020)
Speakers:
Dr. Niklas Larsson, Associate Prinicipal Scientist, AstraZeneca
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learnings:
– Understand principles of SPR microscopy.
– See small molecule and antibody binding case studies
– Learn about receptor pharmacology studies on GPR39 via cellular assay
– Demonstrate target engagement for membrane receptors including presentation of GPR39 SPRM data.
Cell-based Label-free Binding Affinity and Kinetics Using SPR Microscopy Technology
Cambridge Healthtech Institute webinar (November 6 2019)
Speakers:
Late Prof Nongjian “NJ” Tao, PhD, Center Director and Professor, Biodesign Center for Bioelectronics and Biosensors, Arizona State University
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learnings:
– Understand principles of SPR microscopy.
– See application examples developed with Pfizer, AstraZeneca, and others.
– Explore SPRM studies of HER2 (human epidermal growth factor receptor 2) and antibody interactions.
– Discuss detection of single exosomes using an enhanced SPR microscope set up.
表面等离子体基元共振细胞分析仪: 原理与应用
报告人：武仕杰, Applications Scientist, Biosensing Instrument
专门针对细胞表面膜蛋白及相关分子无标记检测而设计的SPRM 200, 使您在不需要提取分离膜蛋白的前提下实时观测细胞状态并同时测量药物分子与靶点在细胞上的动力学结合过程。
Introduction of Electrochemistry SPR
Speakers:
Dr. Shijie Wu, Applications Scientist, Biosensing Instrument
Key learnings:
– Basic of EC SPR
– Application examples of EC-SPR and EC-DualFlow Analysis modules