High-quality, efficient, streamlined service
- Work directly with knowledgeable PhD scientists.
- Measure with high-performance SPRm 200 platform.
- Maintain rigorous quality control with high reproducibility and data integrity.
- Provide a Fast turnaround time.
- Have flexibility to meet your unique requirements.
Cell-based assay with SPRm 200
- Real-time, in-vitro, label-free kinetic measurements with simultaneous optical imaging for quantitative binding activity mapping on whole cells.
- Native binding kinetic profile of small molecules and biologics with various modalities for drug delivery designs.
- Validated across numerous cell lines like HEK 293,CHO, SKBR3, SH-EP1, SKBR3, JIMT-1,HeLa, BxPC3, Ovcar3 and Ramos B.
- Binding heterogeneity and avidity analysis.
Comparison with other technologies
Simple and straightforward process
1) Cell Sample Preparation and Seeding:
Culture the cells, grow them on a sensor surface.
2) On cell measurements:
Measure the interaction between drug compounds and cell membrane proteins with simultaneous optical imaging.
3) Binding kinetic profile, heterogeneity and avidity analysis:
Perform kinetic analysis; map binding activities on cell image; evaluate heterogeneity and avidity.
4) Deliverables:
- Detailed report of binding kinetics analysis.
- The description of methods and results.
- Kinetic and affinity constants (ka, kd, KD) for each compound.