Streamlined measurement
Efficient, high-quality customer service
Our experienced PhD application scientists will work with you directly. With an innovative, high-performance detection system, we provide customers with fast turnaround times, strict quality control, and high data integrity.
SPRM measurement with
- Real-time, in-vitro, label-free kinetic measurements with simultaneous optical imaging for quantitative binding activity mapping on whole cells
- Native binding kinetic profile of small molecules and biologics with various modalities for drug delivery designs
- Binding heterogeneity and avidity analysis
Comparison with commonly used methods
|
Criteria |
Radioligand |
FACS |
SPR Microscopy |
|
Sample Processing |
Multistep |
Multistep |
Single step |
|
Measurement |
Affinity |
Affinity |
Affinity, kon & koff |
|
Readout |
Endpoint |
Endpoint |
Real-time |
|
Detection |
Radiolabel |
Fluorescence |
Direct, label-free |
|
Cell-based |
Yes |
Yes |
Yes |
|
Data |
Average |
Statistic |
Statistic |
|
Throughput |
Low | High | Low |
|
Heterogeneity Analysis |
No | No | Yes |
Simple and straightforward process
1) Cell Sample Preparation and Seeding:
Culture the cells, grow them on a sensor surface
2) On cell measurements:
Measure the interaction between drug compounds and cell membrane proteins with simultaneous optical imaging
3) Binding kinetic profile, heterogeneity and avidity analysis:
Perform kinetic analysis; map binding activities on cell image; evaluate heterogeneity and avidity.
4) Deliverables:
- Detailed report of binding kinetics analysis
- The description of methods and results.
- Kinetic and affinity constants (ka, kd, KD) for each compound.