Application Note 151: Studying Cell Binding Heterogeneity using SPRm 200
Dynamic diseases like cancer become more heterogeneous over the course of the disease. Due to the heterogeneity, the malignant tumor can harbor a collection of cells with different molecular signatures. …
Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
Inflammatory bowel disease (IBD) is a complex, multifactorial inflammatory disease of the gastrointestinal tract resulting in chronic, aberrant mucosal inflammation of the gastrointestinal tract.1,2 The combination of immunologic, environmental, microbiome, …
Application Note 149: The Effect of Cell Fixation on Binding Interaction Kinetics
Membrane proteins play a major role in cell biology and are an important class of receptors targeted by drug candidates being developed in the pharmaceutical industry.1,2 Techniques for studying the …
Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
Chemoattractant receptors and their cognate ligands are promising drug targets for disruption of the tumor progression cascade. Most of these chemoattractant receptors are conventional seven transmembrane-spanning (7TM) G-coupled protein receptors …
Application Note 147: Variations in the Binding Kinetics of Native and Recombinant Protein Receptors
The long and arduous process of drug development involves screening of drug candidates by determining their affinities towards a cellular target and the kinetics of the interactions. A variety of …
Application Note 145: KINETIC MEASUREMENTS OF COVID-19 VIRAL S1 PROTEIN WITH CELLULAR AND RECOMBINANT ACE2 RECEPTORS
The pandemic due to the SARS-CoV-2 continues to evolve and remains an international concern. Covid infection is caused by the air-borne coronavirus 2 (SARS-CoV-2) and its mutants. The viral invasion …
Application Note 144: Single Cell Apoptosis Monitoring with SPRm Impedance
lectrochemical impedance spectroscopy (EIS) technique consists in introducing a perturbation into the sample by means of an AC voltage to an electrode and detecting its current response.
Application Note 143: Monitoring Electrochemical Activities of Single Prussian Blue Nanoparticles with SPRm Electrochemistry
Prussian blue is a pigment that is used to color paints, inks, textiles, and other commercial product. Prussian blue nanoparticles (PBNPs) are nanomaterials that present unique properties and excellent biocompatibility: they can be synthesized in mild conditions and can be derivatized with polymers and/or biomolecules. In addition, with its ability to transfer electrons efficiently and its large redox potential between reduction and oxidation, these nanoparticles result in enzyme-like characteristics which is ideal for biomedicine as therapy and diagnostic agents.
Application Note 142: Small Molecule Binding to Membrane Transporter Using SPRm200
Transporters are large proteins (40–200 kDa) located in the plasma membrane of cells and organelles. They normally span the membrane many times and modulate the transfer of xenobiotics (including nutrients, micronutrients and pharmaceuticals), and endogenous substances such as neurotransmitters, hormones, signaling molecules, vitamins across cellular membranes, tissues or organ barriers.
Application Note 141: SPRM Measurements of Binding Kinetics between Rituximab and CD20 on Live B Cells
Cell-based immunotherapy has gained great attention from researchers and pharmaceutical companies, particularly due to its promise to treat various cancers. Understanding the interaction between drug candidates and targets on the cell membrane is crucial to drug development.