Application Note 176: On-Cell Kinetic Characterization of Antibody–Drug Conjugates Using Two-Well SPR Microscopy
Antibody-drug conjugates (ADCs) combine the targeting specificity of monoclonal antibodies with potent cytotoxic payloads, enabling selective elimination of diseased cells while limiting systemic toxicity. As the clinical landscape of ADCs …
Application Note 175: Highly Sensitive Detection of hCG Using Graphene Oxide-Based Surface Plasmon Resonance Aptamers
There is a growing need for enhanced diagnostic technologies to provide high sensitivity, selectivity, and reproducibility in biosensors. Human chorionic gonadotropin (hCG), a relevant clinical biomarker linked with fetal defects, …
Application Note 174: Structure-Dependent ECD Anchoring of GLP-1R by Therapeutic Peptides
GLP-1R is a class B GPCR centrally involved in glucose homeostasis, insulin secretion, appetite, and β-cell maintenance and thus stands as one of the most important targets in metabolic disease …
Application Note 173: Label-Free Kinetic Analysis of Carbonic Anhydrase IX Inhibitors on Live Suspension Cells Using Surface Plasmon Resonance Microscopy
Transmembrane carbonic anhydrase IX (CA IX) is a zinc metalloenzyme and overexpressed in numerous cancers and represents an attractive therapeutic target due to its limited expression in normal tissues and …
Application Note 172: Affinity and Avidity Kinetic Binding Analysis Using SPRM
Surface Plasmon resonance microscopy (SPRM) enables high-resolution evaluation of the kinetic binding heterogeneity on whole single cells, without disrupting the native environment of the target. This capability permits measurement in …
Application Note 171: Probing the Role of Dissolved CO₂ in Electrochemical Interfaces via EC SPR
Carbon dioxide (CO₂) capture is a key approach to addressing the present climate crisis because of over emission of greenhouse gases. As the dominant human-made cause of global warming, CO₂ …
Application Note 170: Simultaneous Kinetic Analysis of HER2 Binding in Pancreatic and JIMT-1 Cancer Cells Using SPRm 220
HER2 (Human Epidermal Growth Factor Receptor 2) is an important therapeutic target in different types of cancer because it functions to advance tumor development and growth.1 Antibody-based drugs such as …
Application Note 169: Simultaneous Kinetic Binding Analysis of Anti-Mucin-4 on Pancreatic Cancer and HEK293T Cells Using Two-well SPR Microscopy Chip
Mucin 4 (MUC4) is a highly glycosylated cell surface protein that is highly overexpressed in cancerous cells of the pancreas, including the BxPC3 cell line. 1, 2 Due to its tumor-specific expression and cell surface localization, MUC4 is …
Application Note 168: Enhanced SPR Detection of Small Molecules and Bacterial Markers Using Directionally Immobilized Aptamers on Engineered Nanoplatforms
Aptasensors have rapidly become informative tools in modern diagnostics due to their fast response, excellent specificity, and readability for a large range of complex samples. Biosensors exploit the unique molecular …
Application Note 167: Label-Free Characterization of Antibody-Receptor Interactions on Live Suspension Cells using SPRM
Antibody drug development is emerging as the cornerstone of modern therapeutics, particularly in the realm of immunotherapy. Antibodies have demonstrated great efficacy in treating B-cell cancers by taking advantage of …