Application Note 182: Label-Free Kinetic Analysis of Anti-TCR Binding to Jurkat T Cells Using Surface Plasmon Resonance Microscopy
Understanding how antibodies interact with cell surface receptors is fundamental to the development of immunotherapies, T-cell engagers, and other biologic drugs. Flow cytometry is widely used to characterize antibody binding …
Application Note 181: In Vitro Characterization of Agonist and Antagonist Peptide Binding Interaction Kinetics to GLP-1R in HEK293T Cells Using SPR Microscopy
The glucagon-like peptide-1 receptor (GLP-1R), a class B G protein-coupled receptor (GPCR), is a validated therapeutic target for type 2 diabetes and obesity owing to its central role in glucose …
Application Note 179: Resolving Multiple Kinetic Binding Modes in Complex Biological Systems
Surface Plasmon Resonance Microscopy (SPRM) can directly resolve multiple kinetic binding modes in heterogeneous biological samples by extracting independent sets of kinetic parameters from spatially defined regions of interest (ROIs). …
Application Note 178: Modality-Dependent Binding to GLP-1R on Live Cells
The glucagon like peptide-1 receptor (GLP-1R) is a class B G-protein-coupled receptor (GPCR) and one of the most therapeutically important targets in metabolic disease. Its activation promotes insulin secretion, reduces …
Application Note 177: Integrated evaluation of payload-, Fc-, and target-mediated mechanisms of ADCs using streamlined complementary platforms
The poster presented by Alpana Prasad of Eurofins DiscoverX during the AACR annual meeting 2026; now available for download. Authors: Alpana Prasad1, Surekha Bonasu1, Radhika Venkatnarayanan1, Jennifer Lin-Jones1, Jane E. …
Application Note 176: On-Cell Kinetic Characterization of Antibody–Drug Conjugates Using Two-Well SPR Microscopy
Antibody-drug conjugates (ADCs) combine the targeting specificity of monoclonal antibodies with potent cytotoxic payloads, enabling selective elimination of diseased cells while limiting systemic toxicity. As the clinical landscape of ADCs …
Application Note 173: Label-Free Kinetic Analysis of Carbonic Anhydrase IX Inhibitors on Live Suspension Cells Using Surface Plasmon Resonance Microscopy
Transmembrane carbonic anhydrase IX (CA IX) is a zinc metalloenzyme and overexpressed in numerous cancers and represents an attractive therapeutic target due to its limited expression in normal tissues and …
Application Note 172: Affinity and Avidity Kinetic Binding Analysis Using SPRM
Surface Plasmon resonance microscopy (SPRM) enables high-resolution evaluation of the kinetic binding heterogeneity on whole single cells, without disrupting the native environment of the target. This capability permits measurement in …
Application Note 170: Simultaneous Kinetic Analysis of HER2 Binding in Pancreatic and JIMT-1 Cancer Cells Using SPRm 220
HER2 (Human Epidermal Growth Factor Receptor 2) is an important therapeutic target in different types of cancer because it functions to advance tumor development and growth.1 Antibody-based drugs such as …
Application Note 169: Simultaneous Kinetic Binding Analysis of Anti-Mucin-4 on Pancreatic Cancer and HEK293T Cells Using Two-well SPR Microscopy Chip
Mucin 4 (MUC4) is a highly glycosylated cell surface protein that is highly overexpressed in cancerous cells of the pancreas, including the BxPC3 cell line. 1, 2 Due to its tumor-specific expression and cell surface localization, MUC4 is …