Application Note 167: Label-Free Characterization of Antibody-Receptor Interactions on Live Suspension Cells using SPRM
Antibody drug development is emerging as the cornerstone of modern therapeutics, particularly in the realm of immunotherapy. Antibodies have demonstrated great efficacy in treating B-cell cancers by taking advantage of …
Application Note 165: Spatially Resolved Detection of Electroactive Biological Systems Using EC-SPR Microscopy
Traditional electrochemical (EC) techniques typically quantify averaged responses from a collection of individual elements and molecules on the surface of electrode.1 These approaches, while effective for bulk analysis, often overlook …
Application Note 163: Binding Activities of Yohimbine Analogues on ADRA2A Overexpressing Live Cells
Millions of sepsis-related deaths are reported every year, which makes acute septic shock one of the leading causes of death in intensive care units around the world.1 Septic shock is …
Application Note 162: N-linked Glycan Deglycosylation Unmasks New HPA O-linked Glycan Heterogeneity
Most membrane proteins carry some form of glycans, which regulate many cellular processes such as cell differentiation and cell-to-cell interactions.1 There are two types of glycans based on the type …
Application Note 160: Influence of Aberrant Glycosylation on the Binding Capability of Muc-4 in Pancreatic Cancer Cells
Mucin-4 (Muc-4) is a heavily glycosylated membrane glycoprotein which is associated with pancreatic cancer and metastasis.1 This glycoprotein primarily has O-glycans which contributes to its bulky structure in the extracellular …
Application Note 159: Real-Time Monitoring of Glycan Heterogeneity in Cancer Cells
Membrane proteins are the main targets of therapeutic drugs and most of them carry some form of glycan structures, which play pivotal roles in protein folding, stability, cell communication, cell …
Application Note 158: Minocycline Enhanced T-cell Cytotoxicity of Tumor Cells
Cancer cells propagate by finding means to hide from the immune system. Immunotherapies allow the immune system to recognize and attack the hidden cancer cells reducing the spread of the …
Application Note 157: Understanding the Glyco-code using SPR microscopy
Incomplete synthesis of glycans or the overexpression of certain glycans promote a tumor-specific glyco-code.1 The glyco-code includes increased branched N-glycans, truncated O-glycans, hyper-sialylated glycans or glycolipids.2&3 Non-catalytic glycan binding proteins …
Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
HER2 also known as ErbB2 is an epidermal growth factor receptor and is a 185 kDa transmembrane glycoprotein. 20–30% of breast cancer patients have overexpression of HER2.1 The extracellular domain …
Application Note 153: Exploring Affinity, Avidity, and Valency using SPRM
The accurate characterization of binding kinetics of therapeutic antibodies to tumor cells is critical to advancing therapeutic drug development.1,2,3 SPR microscopy (SPRM) is a label free technology utilized for studying …