Application Note 158: Minocycline Enhanced T-cell Cytotoxicity of Tumor Cells
Cancer cells propagate by finding means to hide from the immune system. Immunotherapies allow the immune system to recognize and attack the hidden cancer cells reducing the spread of the …
Application Note 157: Understanding the Glyco-code using SPR microscopy
Incomplete synthesis of glycans or the overexpression of certain glycans promote a tumor-specific glyco-code.1 The glyco-code includes increased branched N-glycans, truncated O-glycans, hyper-sialylated glycans or glycolipids.2&3 Non-catalytic glycan binding proteins …
Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
HER2 also known as ErbB2 is an epidermal growth factor receptor and is a 185 kDa transmembrane glycoprotein. 20–30% of breast cancer patients have overexpression of HER2.1 The extracellular domain …
Application Note 153: Exploring Affinity, Avidity, and Valency using SPRM
The accurate characterization of binding kinetics of therapeutic antibodies to tumor cells is critical to advancing therapeutic drug development.1,2,3 SPR microscopy (SPRM) is a label free technology utilized for studying …
Application Note 151: Studying Cell Binding Heterogeneity using SPRm 200
Dynamic diseases like cancer become more heterogeneous over the course of the disease. Due to the heterogeneity, the malignant tumor can harbor a collection of cells with different molecular signatures. …
Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
Inflammatory bowel disease (IBD) is a complex, multifactorial inflammatory disease of the gastrointestinal tract resulting in chronic, aberrant mucosal inflammation of the gastrointestinal tract.1,2 The combination of immunologic, environmental, microbiome, …
Application Note 149: The Effect of Cell Fixation on Binding Interaction Kinetics
Membrane proteins play a major role in cell biology and are an important class of receptors targeted by drug candidates being developed in the pharmaceutical industry.1,2 Techniques for studying the …
Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
Chemoattractant receptors and their cognate ligands are promising drug targets for disruption of the tumor progression cascade. Most of these chemoattractant receptors are conventional seven transmembrane-spanning (7TM) G-coupled protein receptors …
Application Note 147: Variations in the Binding Kinetics of Native and Recombinant Protein Receptors
The long and arduous process of drug development involves screening of drug candidates by determining their affinities towards a cellular target and the kinetics of the interactions. A variety of …
Application Note 145: KINETIC MEASUREMENTS OF COVID-19 VIRAL S1 PROTEIN WITH CELLULAR AND RECOMBINANT ACE2 RECEPTORS
The pandemic due to the SARS-CoV-2 continues to evolve and remains an international concern. Covid infection is caused by the air-borne coronavirus 2 (SARS-CoV-2) and its mutants. The viral invasion …