Application Note 157: Understanding the Glyco-code using SPR microscopy
Incomplete synthesis of glycans or the overexpression of certain glycans promote a tumor-specific glyco-code.1 The glyco-code includes increased branched N-glycans, truncated O-glycans, hyper-sialylated glycans or glycolipids.2&3 Non-catalytic glycan binding proteins …
Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
HER2 also known as ErbB2 is an epidermal growth factor receptor and is a 185 kDa transmembrane glycoprotein. 20–30% of breast cancer patients have overexpression of HER2.1 The extracellular domain …
Application Note 155: Rapid Determination of IgG in Sera using BI-4500A SPR System
IgG deficiencies could be a major reason for impaired humoral immunity, whereas very high IgG levels could be a result of chronic infections, autoimmune disorders, and allergies.1 The levels of …
Application Note 154: Determination of Active Concentration Using Calibration-free Methods
Kinetics of an antibody-antigen interaction forms the basis for immunological response and antibody therapeutics,
which is one of the fastest areas of growth in the pharma industry. Affinity and avidity of antibodies are the characteristics which define the overall efficacy of binding kinetics.
Application Note 153: Exploring Affinity, Avidity, and Valency using SPRM
The accurate characterization of binding kinetics of therapeutic antibodies to tumor cells is critical to advancing therapeutic drug development.1,2,3 SPR microscopy (SPRM) is a label free technology utilized for studying …
Application Note 152: Binding Studies of an Integrin and its Mutants using BI-4500
Integrins are heterodimeric receptors which help in cell adhesion and are ubiquitous to all metazoans.1 They also play a vital role in numerous cellular processes including cell growth, proliferation, differentiation, …
Application Note 151: Studying Cell Binding Heterogeneity using SPRm 200
Dynamic diseases like cancer become more heterogeneous over the course of the disease. Due to the heterogeneity, the malignant tumor can harbor a collection of cells with different molecular signatures. …
Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
Inflammatory bowel disease (IBD) is a complex, multifactorial inflammatory disease of the gastrointestinal tract resulting in chronic, aberrant mucosal inflammation of the gastrointestinal tract.1,2 The combination of immunologic, environmental, microbiome, …
Application Note 149: The Effect of Cell Fixation on Binding Interaction Kinetics
Membrane proteins play a major role in cell biology and are an important class of receptors targeted by drug candidates being developed in the pharmaceutical industry.1,2 Techniques for studying the …
Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
Chemoattractant receptors and their cognate ligands are promising drug targets for disruption of the tumor progression cascade. Most of these chemoattractant receptors are conventional seven transmembrane-spanning (7TM) G-coupled protein receptors …