Application Note 168: Enhanced SPR Detection of Small Molecules and Bacterial Markers Using Directionally Immobilized Aptamers on Engineered Nanoplatforms
Aptasensors have rapidly become informative tools in modern diagnostics due to their fast response, excellent specificity, and readability for a large range of complex samples. Biosensors exploit the unique molecular …
Application Note 167: Label-Free Characterization of Antibody-Receptor Interactions on Live Suspension Cells using SPRM
Antibody drug development is emerging as the cornerstone of modern therapeutics, particularly in the realm of immunotherapy. Antibodies have demonstrated great efficacy in treating B-cell cancers by taking advantage of …
Application Note 166: Probing Redox Interactions Between Microbial EPS and p-Nitrophenol Using Electrochemical Surface Plasmon Resonance
Microbial extracellular polymeric substances (EPS) with redox functional groups play a vital role in the bioconversion of pollutants, which can affect their reactivity toward diverse environmental pollutants. However, the redox …
Application Note 165: Spatially Resolved Detection of Electroactive Biological Systems Using EC-SPR Microscopy
Traditional electrochemical (EC) techniques typically quantify averaged responses from a collection of individual elements and molecules on the surface of electrode.1 These approaches, while effective for bulk analysis, often overlook …
Application Note 164: Simultaneous Analysis of Binding Interactions using Multi-Channel SPR
Entry level surface plasmon resonance (SPR) tools with dual channels have only one measurement point since the other channel is always used as a reference.1 Therefore, increasing efforts have been …
Application Note 163: Binding Activities of Yohimbine Analogues on ADRA2A Overexpressing Live Cells
Millions of sepsis-related deaths are reported every year, which makes acute septic shock one of the leading causes of death in intensive care units around the world.1 Septic shock is …
Application Note 162: N-linked Glycan Deglycosylation Unmasks New HPA O-linked Glycan Heterogeneity
Most membrane proteins carry some form of glycans, which regulate many cellular processes such as cell differentiation and cell-to-cell interactions.1 There are two types of glycans based on the type …
Application Note 161: Surface Plasmon Resonance Identifies Lysozyme Interaction with Tannic Acid
Lysozyme (LZM) is found in body secretions like saliva, and it can catalytically hydrolyze peptidoglycans and exert catalysis-independent antimicrobial properties.1 Additionally, it is a valuable component in pharmaceutical and food …
Application Note 160: Influence of Aberrant Glycosylation on the Binding Capability of Muc-4 in Pancreatic Cancer Cells
Mucin-4 (Muc-4) is a heavily glycosylated membrane glycoprotein which is associated with pancreatic cancer and metastasis.1 This glycoprotein primarily has O-glycans which contributes to its bulky structure in the extracellular …
Application Note 159: Real-Time Monitoring of Glycan Heterogeneity in Cancer Cells
Membrane proteins are the main targets of therapeutic drugs and most of them carry some form of glycan structures, which play pivotal roles in protein folding, stability, cell communication, cell …