High-quality, efficient, and streamlined services
- Work directly with SPRm 200 experts
- Quick turn around
- Rigorous quality control to develop robust methods
Simple and straightforward process
Our deliverables:
- Report of binding kinetics analysis (KD, kon, koff) for each binding experiment and description of experimental methods.
- Develop dose response methods (6-8 concentrations over a suitable concentration range).
- Optimize experimental assay.
Required from customer:
- Analyte(s)
- Cells grown on the SPRm cell chamber sensor chip
Kinetics of anti-HER2 binding to extracellular domain II of HER2 with its negative control studies
SPRm cell chip: SKBR3 (positive control) and HEK293 (negative control)
Analyte: anti-HER2 solutions at 3.15, 6.25, 12.5, 25, 50, and 100 nM
SKBR3 (positive control) measurement data:
Right: Bright-field image of SKBR3. Left: corresponding SPR image of the SKBR3 cells with red squares representing areas of binding activity
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Histograms of the kinetic parameters (KD, ka and kd) were fitted with Gaussian distributions to extract the mean and 95% confidence interval of the cell population. KD of 4.91 nM, ka of 1.43 x 106 M-1s-1 and kd of 5.63 x 10-3 s-1.
HEK293 (negative control) measurement data:
Right: Bright-field image of HEK293. Left: corresponding SPR image of the HEK293 cells. Lack of red squares represents negligible binding.