Bibliography
…separation and binding kinetics analysis.” Journal of The Electrochemical Society 169.5 (2022): 057509. Jawad, B., Adhikari, P., Cheng, K., Podgornik, R., Ching, W.-Y., “Computational Design of Miniproteins as SARS-CoV-2 Therapeutic…
Biosensing Instrument is a Fall 2019 Arizona Innovation Challenge awardee
…at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA) is the state’s leading economic development organization with a streamlined mission to grow and strengthen Arizona’s economy. The ACA…
Biosensing Instrument named “Best Specialty Life Science Instruments Supplier” in Global Health & Pharma’s 2020 Biotechnology Awards
…our full winner listing, please visit the GHP website. GHP’s official press release can be accessed at https://www.ghp-news.com/ghp-news-2020-biotechnology-awards-winners-press-release/ About Biosensing Instrument Biosensing Instrument, Inc. (BI) is a privately held company based…
BIOSENSING INSTRUMENT EXHIBITING AT Drug DISCOVERY Chemistry 2024
…will exhibit at Drug Discovery Chemistry 2024 on April 1st-4th, 2024 in San Diego, California. Please visit us at booth 511, For more information about this conference, go to https://www.drugdiscoverychemistry.com…
BIOSENSING INSTRUMENT EXHIBITING AT DISCOVERY ON TARGET 2024
…exhibit at Discovery on Target 2024 on October 1 – 3, 2024 in Boston, Massachusetts. Please visit us at booth 421, For more information about this conference, go to https://www.discoveryontarget.com/…
Biosensing Instrument
…2024 Posted on Aug 03 2024 BIOSENSING INSTRUMENT EXHIBITING AT Drug DISCOVERY Chemistry 2024 Posted on Mar 26 2024 ON DEMAND CELL-BASED SPRm WEBINAR WITH Dr. Rives Posted on Oct…
We are hiring!
Come and join us to be part of an exciting company designing cutting edge technology and award winning products for the early drug discovery market! We are looking for a Technical…
Biosensing Instrument exhibiting at the 2019 Antibody Engineering & Therapeutics conference
…will be exhibited at the conference. SPRm 200 is the world’s first commercially available surface plasmon resonance microscopy (SPRM) system and it is the only scientific solution on the market…
Application Note 150: Studying the role of an antagonist for a pH-sensing GPCR using SPRm 200
…profile.6 Despite reasonable exposures consistent with previous reports, Compound 13 was ineffective at preventing colitis in IL10-/- mice.7 The lack of apparent and significant target engagement led to investigating the…
Arizona Commerce Authority Announces the Fall 2019 Arizona Innovation Challenge Finalists
…Application areas include life sciences, drug discovery, antibody characterization, biosensors and electrochemistry. Information about BI can be found at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA)…
Application Note: 107 – Binding Kinetics Analysis with SPR: Interaction between Bovine Serum Albumin (BSA) and Anti-BSA
…expedite the experimental process leading to higher quality results faster. Author: Nguyen Ly | Biosensing Instrument | Published April 15, 2015 DOWNLOAD PDF Download a PDF of Application Note: 107…
Biosensing Instrument is a Semifinalist in the Fall 2019 Arizona Innovation Challenge
…of panels and workshops within the subsequent 12 months of the award. Media Contact Connie Weber, Arizona Commerce Authority, conniew@azcommerce.com About the Arizona Commerce Authority The Arizona Commerce Authority (ACA)…
Contact Us
…1007 E Warner Rd #102 Tempe, AZ 85284 +1 480-491-2777 We have a large distribution network around the world! Contact us for more information. CONTACT SALES info2BI@Biosensinginstrument.com CONTACT SUPPORT support@Biosensinginstrument.com…
Application Note 101: Fast Kinetics of Conformational Changes in Proteins
…Ly | Biosensing Instrument | Published January 1, 2008 DOWNLOAD PDF Download a PDF of Application Note 101: Fast Kinetics of Conformational Changes in Proteins Download Now Note References S….
Application Note 160: Influence of Aberrant Glycosylation on the Binding Capability of Muc-4 in Pancreatic Cancer Cells
…complex influences of the native N-glycan cellular environment on binding interactions to the Muc-4 receptor as this is currently the only commercially available label-free technique with high enough sensitivity and…
Application Note 132: SR142948 Antagonist Binding to NTS-1 Receptor Using SPR Microscopy
…Dr. Scott Pollack from Sygnature Discovery for the samples, data and valuable insights. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of…
Application Note 123: Surface Plasmon Resonance Measurement of Protein-Peptide Interaction Using Streptavidin Sensor Chip
…Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 123: Surface Plasmon Resonance Measurement of Protein-Peptide Interaction Using Streptavidin Sensor Chip Download Now Note…
Application Note 131: SPR Microscopy for Live Suspension Cells
…the samples and valuable discussions. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 131: SPR Microscopy for Live Suspension…
Application Note: 108 – SPR as a Chromatographic Detector: Separation and Label-Free Detection of Biomolecules
…electrophoresis and with microfluidic separations are just two of such possibilities. Author: Nguyen Ly | Biosensing Instrument | Published May 1, 2016 DOWNLOAD PDF Download a PDF of Application Note:…
Application Note 104: Flow-Injection SPR: Sensitive Determinations of Heavy Metal Ions
…2010 DOWNLOAD PDF Download a PDF of Application Note 104: Flow-Injection SPR: Sensitive Determinations of Heavy Metal Ions Download Now Note References Chah, S.; Yi, J.; Zare, R. N. Sens….
Application Note 122: Surface Plasmon Resonance Microscopy for Multiple and Single Cell Membrane Binding Kinetics Studies
…on a single cell. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 122: Surface Plasmon Resonance Microscopy for Multiple…
Application Note 137: Ginnalin A and Tabersonine Inhibition of Amyloid β(1-42) Aggregation
…on neurons (3). In this study, the binding interactions of two natural compounds with Αβ(1-42) were measured. Ginnalin A (GA), a phenolic compound isolated from the red maple (Acer rubrum)…
Application Note 103: Electrochemical SPR: Metal Deposition/Stripping and Reorganization of Electroactive Organic Thin Films
…Ly | Biosensing Instrument | Published May 1, 2010 DOWNLOAD PDF Download a PDF of Application Note 103: Electrochemical SPR: Metal Deposition/Stripping and Reorganization of Electroactive Organic Thin Films Download…
Application Note: 113 – Flow-Through Electrochemical Surface Plasmon Resonance (SPR): Detection of Intermediate Reaction Products
…HQ-BQ at position 3. Flow rate = 100 µL/min. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note: 113 –…
Application Note 117: Real-time Monitoring Biomarker Expression of Carcinoma Cells
…and antineoplastic drug development. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 117: Real-time Monitoring Biomarker Expression of Carcinoma…
Application Note 119: Small Molecule Detection by Surface Plasmon Resonance (SPR)
…disorders, and discover antifungal and antibacterial agents. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 119: Small Molecule Detection…
Application Note 127: Quantifying Antibody Binding to Membrane Proteins on Single Cells with SPR Microscopy
…| Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 127: Quantifying Antibody Binding to Membrane Proteins on Single Cells with SPR Microscopy Download Now Note References…
Application Note 106: Flow Injection-SPR: Sensitive and Sequence-Specific DNA Assays
…amplification scheme. Author: Nguyen Ly | Biosensing Instrument | Published June 11, 2014 DOWNLOAD PDF Download a PDF of Application Note 106: Flow Injection-SPR: Sensitive and Sequence-Specific DNA Assays Download…
Technical Note 102: SPR Sensitivity and Detection Limit
…of sensitivity between units of degree angular shift and RIU requires careful consideration of instrumental conditions. Example 5 How do the following SPR instruments compare? The first instrument has a BK7…
Technical Notes
…Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for studying binding behavior of biomolecules. Since its commercialization in 1990’s [1], SPR has made vast advances in terms…
Application Note 133: Interaction of Protein Phosphatase 1 with its Muscle Glycogen– targeting Regulatory Subunit Measured by SPR
…was measured with the help from Biosensing Instruments researchers. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 133: Interaction…
Application Note 121: Protein Interactions in the Mycofactocin Biosynthetic Pathway
…KD (C). Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 121: Protein Interactions in the Mycofactocin Biosynthetic Pathway Download…
Application Note 156: Kinetics of Anti-HER2 Binding to Extracellular Domain II of HER2
…epitope space of HER2 for potential clinical targets in a systematic manner. Surface plasmon resonance microscopy (SPRM) using BI’s SPRm200, integrates label-free, high-resolution SPR binding kinetic measurements with bright-field optical…
Application Note 126: GPCR Binding Assays with SPR Microscopy
…Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 126: GPCR Binding Assays with SPR Microscopy Download Now Note References Locatelli-Hoops, S et al,…
Application Note 128: SPR Microscopy for Acid-Sensing Ion Channels
…| Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 128: SPR Microscopy for Acid-Sensing Ion Channels Download Now Note References Lingueglia, E et…
Application Note 147: Variations in the Binding Kinetics of Native and Recombinant Protein Receptors
…a broad range of binding interaction behaviors that should not be treated homogeneously by simply averaging the behaviors of the entire cell population together, as is the common practice 13….
Application Note 146: ENHANCED REDUCTION OF NONSPECIFIC INTERACTIONS
…region in which cells were permitted to grow freely. The red square indicates an example area for detection that includes both cell regions and FBS-free regions for referencing. III. Data…
SPRm 200 Series
…measurement of phenotypical changes of the sample via bright field and binding strength and kinetics via SPR. Click here to download a PDF datasheet. Key Features System Specifications References Integrated…
Biosensing Instrument graduates the Venture Ready Accelerator program
…electrochemistry. Information about BI can be found at www.biosensingUSA.com. About the Arizona Commerce Authority The Arizona Commerce Authority (ACA) is the state’s leading economic development organization with a streamlined mission…
FCOI Policy
…applications for Phase I support under the Small Business Innovation Research (SBIR) and Small Business Technology Transfer (STTR) programs. Compliance and Penalties for Non-Performance If an investigator fails to comply…
Application Note 124: Quantifying Molecular binding to Membrane Proteins on Individual Cells with Surface Plasmon Resonance Microscopy
…Download a PDF of Application Note 124: Quantifying Molecular binding to Membrane Proteins on Individual Cells with Surface Plasmon Resonance Microscopy Download Now Note References Dell, A. et al, Science,…
Application Note 125: Lectin-Glycoprotein Interactions with SPR Microscopy
…Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 125: Lectin-Glycoprotein Interactions with SPR Microscopy Download Now Note References Patching, S.,…
Application Note: 109 – Application of SPR to Bacteriology: Endotoxin/Protein Interaction Studies
…for his technical help, discussion, and the provision of the CD-14 protein and endotoxin samples. DOWNLOAD PDF Download a PDF of Application Note: 109 – Application of SPR to Bacteriology:…
Application Note 120: Electrochemical SPR Monitoring of Drug Interactions with Live Cancer Cells
…and SPR techniques. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 120: Electrochemical SPR Monitoring of Drug Interactions with…
Application Note: 110 – Studying Protein Adsorption Properties with SPR
…We would like to thank Dr. Chae for sharing his results with us. DOWNLOAD PDF Download a PDF of Application Note: 110 – Studying Protein Adsorption Properties with SPR Download…
Accessories
…AUTOMATIC BUFFER EXCHANGE PUMP WITH DEGASSER Automatic buffer exchange up to six sources with in-line degasser. Download PDF BI-AUTOSAMPLER Automate your sample delivery in a high throughput fashion. Download PDF…
Application Note 140: Membrane Transport Protein Binding Kinetics Using Label-Free SPRm200
…obtain using traditional labelled and label-free methods. Measuring biophysical properties in the membrane target’s native environment in real time and label free provides insightful drug-receptor interactions information much earlier in…
Application Note 139: Single-Injection Kinetic Measurement Technique for Studies of COVID-19 S1 Protein and ACE2 Binding
…can be seriously compromised as the “regeneration” step is not reliable or simply cannot be performed. Even with ligands that can be completely recovered, injections of multiple samples upon repeated…
Privacy Policy
…subscription (such as e-mail newsletters) Content submissions, community postings (i.e. forums or bulletin boards), comments and suggestions We and our affiliates and vendors may use personal information about you to…
BI-4500 Series
…for advanced five-channel ultra-fast kinetics research Optional Accessories: EC-DualFlow™ Module for advanced two-channel electrochemical (EC) flow SPR research EC-SPR Module for electrochemical (EC) SPR research Gas SPR Module for gas…
BI-2500 Series
Ideal system for developing biosensors in your research. Its flexible design allows an upgrade path for electrochemical SPR or chemical vapor (gas) SPR combination studies. Click here to download a…
Application Note: 112 – Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell Lysates
…Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note: 112 – Detection of Wild-Type and Mutant p53 Proteins in Cancer Cell…
Application Note: 105 – Protein/Drug Interaction: Ferulic Acid and Bovine Serum Albumin
…0.4, 1.0 µM) were injected. A flow rate of 80 µL/min was used. Author: Nguyen Ly | Biosensing Instrument | Published July 1, 2012 DOWNLOAD PDF Download a PDF of…
Application Note 102: Electrochemical SPR: Redox-Induced Protein Conformational Changes
…Ly | Biosensing Instrument | Published April 1, 2009 DOWNLOAD PDF Download a PDF of Application Note 102: Electrochemical SPR: Redox-Induced Protein Conformational Changes Download Now Note References S. Boussaad,…
Application Note 114: SPR Binding Affinity Determination of Novel Peptide Inhibitors to the Innate Immune activator C1q
…to remove immune complexes and apoptotic cells. Three different pathways comprise the complement system: the classical, lectin and alternative pathways [1]. While the complement system plays a central role in…
Application Note 115: SPR Assay of Clinical Alzheimer Disease Samples: Amyloid β Peptides in Cerebrospinal Fluids
…Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 115: SPR Assay of Clinical Alzheimer Disease Samples: Amyloid β Peptides in Cerebrospinal Fluids Download Now Note References…
Application Note 116: Temperature Dependence of Enzymatic Cleaving Activity
…an important feature for SPR measurements. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 116: Temperature Dependence of Enzymatic…
Application Note 118: SPR for Food Safety and Monitoring: Melamine in Milk Samples
…performed for simple, reliable, and fast testing of melamine in milk. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note…
Application Note 130: Measuring molecular binding kinetics with SPRM Impedance
…are shown on the graphs. Applied voltage: 0.3 V amplitude at 5 Hz. Buffer: PBS. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a…
Application Note 129: AZ1395 small molecule targeting GPR39
…Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 129: AZ1395 small molecule targeting GPR39 Download Now Note References Tang,…
Application Note: 111 – Measuring Surface Charge Density: A New Application with SPR
…are caused by surface regeneration. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note: 111 – Measuring Surface Charge Density:…
Application Note 154: Measuring Affinity and Avidity under High and Low Expression Conditions
…binding of Herceptin to SKBR3 (high HER2 expressing cell line) and (B) bivalent binding of Herceptin to MCF-7 (low HER2 expressing cell line). The SPRm 200 system combines bright field…
Application Note 157: Understanding the Glyco-code using SPR microscopy
…kinetics of these heterogenous glycan-lectin interactions have remained understudied due to the lack of efficient in vitro, label-free biophysical tools.3 In this application note, we study the interactions of glycans…
Application Note 155: Rapid Determination of IgG in Sera using BI-4500A SPR System
…IgG in the sera are measured to understand the immune function in patients.2 Clinical assays of total IgG molecules in sera are commonly measured with cellulose acetate membrane electrophoresis3 or…
Free webinar on May 25 2021: Applications of EC-SPR and SPRm Impedance
Biosensing Instrument will be hosting a free webinar in collaboration with California State University, Los Angeles and Admiral Instruments on May 25 2021, 9am LA / noon NY / 5pm…
Biosensing Instrument speaking and exhibiting at the 2020 Discovery on Target Virtual conference
…only scientific solution in the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins directly on whole cells. For more information about the conference: https://www.discoveryontarget.com/…
Biosensing Instrument attending 9th NovAliX Conference
…will present at #novalixconference on Kinetic Characterization of ligand and antibody binding to cell surface expressed CCRL2 using #SPRm200 at IL01 1:10pm on 4/26/2023. https://www.novalix-conferences.org/programme For more information about the meeting, go to https://www.novalix-conferences.org…
Biosensing Instrument exhibiting at the 19th Discovery on Target conference
…the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.discoveryontarget.com/…
BIOSENSING INSTRUMENT EXHIBITING AT THE 2022 Drug Discovery Chemistry Conference
…unique scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.drugdiscoverychemistry.com/…
BIOSENSING INSTRUMENT EXHIBITING AT Drug Discovery Chemistry 2023
…unique scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the meeting, go to https://www.drugdiscoverychemistry.com/programs…
Application Note 159: Real-Time Monitoring of Glycan Heterogeneity in Cancer Cells
…and associated binding interaction kinetics. Also, these SPRm 200 results highlight the changes and the complexity of glycosylation in pancreatic cancer cells with high resolution which is not readily observable…
Free webinar on Jan 28 2021: SPR for Biosensor Applications
Biosensing Instrument will be talking at a free webinar on Jan 28 2021, 8am LA / 11am NY / 4pm London time. SPR is an optical-based real-time detection method that…
Application Note 148: New Discovery of Chemerin Binding to CCRL2 Receptor Using SPRm 200
…-1), off-rate (kd = 1.16E-03 s -1) and binding affinity (KD = 5.49 nM) were reported. Importantly, the mean KD of chemerin binding to huCCRL2 determined by SPRM was comparable…
Application Note 136: Sensitivity and kinetic analysis of carboxyl-graphene oxide-based SPR biosensors for label-free detection of lung cancer biomarker
…a modified Hummer method, yielding a carboxylate product from chloroacetic acid (Cl–CH2–COOH) under basic conditions. A carboxyl-GO sheet has easily synthesized functional groups, which form unique composites (2). Using an assay with…
Life Science
…SPR: Interaction between Bovine Serum Albumin (BSA) and Anti-BSA Application Note: 108 – SPR as a Chromatographic Detector: Separation and Label-Free Detection of Biomolecules Application Note: 109 – Application of…
ON DEMAND CELL-BASED SPRm WEBINAR WITH Dr. Rives
…with a variety of small molecule and antibody binding examples. Topics from this webinar: Overview of SPRm principle Review case studies of small molecule pH-dependent binding of GPR4 Explore label-free…
Small Molecule Detection
…compact optical designs with no moving parts further improve stability and performance. The combination of these design methods creates an instrument that can measure molecules as small as 100 Da….
Application Note 142: Small Molecule Binding to Membrane Transporter Using SPRm200
…free manner. However, measuring membrane proteins binding with the SPR has been a challenge because of the need to immobilize purified receptors on the sensor chip surface. SPR microscopy (SPRm)…
Surface Plasmon Resonance
…such molecular binding studies, i.e. ELISA, QCM, and ITC. However, few encom*]}*pass as many advantages as Surface Plasmon Resonance. SPR enables (1) high sensitivity, (2) label-free detection, (3) real-time monitoring,…
Application Note 143: Monitoring Electrochemical Activities of Single Prussian Blue Nanoparticles with SPRm Electrochemistry
Prussian blue is a pigment that is used to color paints, inks, textiles, and other commercial product (1). Prussian blue nanoparticles (PBNPs) are nanomaterials that present unique properties and excellent…
Biosensing Instrument Exhibiting at the 2021 Antibody Engineering and Therapeutics
…the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://informaconnect.com/antibody-engineering-therapeutics/…
BIOSENSING INSTRUMENT EXHIBITING AT ACS Spring 2023
…label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment. Please visit us at booth 509. For more information about the meeting, go to https://www.acs.org/meetings/acs-meetings/spring-2023.html…
Biosensing Instrument presenting at the 2nd NovAliX Virtual Conference
…solution on the market that enables label-free in vitro measurement of binding affinity and kinetics of cell membrane proteins in their native environment. For more information about the conference, go to https://www.novalix-conferences.org/…
BIOSENSING INSTRUMENT ATTENDING BPS2023
…that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment, Please visit us at Booth 919. For more information, go to https://www.biophysics.org/2023meeting#/…
Webinar with Pfizer: Kinetic characterization of ligand & antibody binding to cell surface expressed CCRL2
…Plasmon Resonance Microscopy) is a powerful technology that enables label-free kinetic binding interaction studies of drug molecules with whole cells in real-time. By combining traditional cell culture and imaging techniques…
Why Surface Plasmon Resonance?
…i.e. ELISA, QCM, and ITC. However, few encom*]}*pass as many advantages as Surface Plasmon Resonance. SPR enables (1) high sensitivity, (2) label-free detection, (3) real-time monitoring, (4) low volume sample…
BIOSENSING INSTRUMENT EXHIBITING AT DISCOVERY ON TARGET 2023
…on #SPRm200, and our research on “Binding interactions to key potential pancreatic cancer vaccine candidate (Mucin-4) are significantly hindered by aberrant glycosylation of pancreatic cancer cell”. For more information about…
End of Support Reminder for BI-2000, BI-3000 and BI-4000 SPR systems
…part orders until December 31, 2020. We are offering trade-in incentives to upgrade to the award winning 5 channel SPR (BI-4500) or the 3 channel SPR (BI-2500). Contact info2bi@biosensingusa.com for more info….
Analysis Modules
…Click here to download a PDF datasheet. FLOW INJECTION SPR Flow injection SPR allows SPR analysis to be carried out in a continuous liquid stream. Read More ELECTROCHEMICAL SPR Electrochemical…
Store
…gasket for EC-DualFlow™ module $579.00 101-00501 O-rings for EC-SPR analysis module (pack of 10) $32.00 106-00604 Complete BSA Starter Kit includes CM Dextran Chip(1), BSA Starter Kit(2) $228.00 106-00605 BSA…
Application Note 152: Binding Studies of an Integrin and its Mutants using BI-4500
…and acts as an important regulator of the immune activities of these cells.4 To date, more than 100 ligands have been reported to bind to Mac-1.2 The binding site for…
BI SPR Technology
At Biosensing Instrument Inc., we use a unique detection method to achieve both high sensitivity and fast detection speeds. Our innovative design and BI-DirectFlow™ technology allows us to combine precise…
Application Note 138: Kinetic Measurement of COVID-19 S1 Protein and ACE2 Binding
…viral attachment to angiotensin (Ang)-converting enzyme 2 (ACE2) (1,2). ACE2, expressed in numerous different tissues, is the receptor through which the COVID-19 spike-protein (S-protein) gains entry into cells for subsequent…
How Does Surface Plasmon Resonance Work?
…oscillations of free electrons (known as surface plasmons) via the Kretschmann configuration, in which light is focused onto a metal film through a glass prism and the subsequent reflection is…
Technical Note 103: Surface Plasmon Resonance v. Quartz Crystal Microbalance
Surface Plasmon Resonance v. Quartz Crystal Microbalance Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM) are two powerful label-free detection techniques that are receiving increasing amounts of attention for…
Technical Note 106: SPR Microscopy vs Radioligand Binding Analysis
…(ka) and dissociation (kd) kinetics. Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for measuring both the equilibrium dissociation constant, and the kinetic constants. Since its commercialization…
SPRm200 webinar with Genentech: Exploration of avidities between monovalent and bivalent antibodies in correlation with cellular expression
…Microscopy) is a novel technology that enables label-free and real-time binding affinity and kinetics of membrane proteins with drug molecules. By combining cell culture and imaging techniques with biophysical assays,…
Application Note 141: SPRM Measurements of Binding Kinetics between Rituximab and CD20 on Live B Cells
Cell-based immunotherapy has gained great attention from researchers and pharmaceutical companies, particularly due to its promise to treat various cancers. Understanding the interaction between drug candidates and targets on the…
On Demand SPRm200 Webinar with Pfizer
Hosted by Labroots, a free SPRm200 webinar with Biosensing Instrument and Pfizer is now available online. In this webinar, Pfizer will show how they utilized SPRM along with other biophysical…
Application Note 149: The Effect of Cell Fixation on Binding Interaction Kinetics
…However, this approach can be too harsh, causing dehydration and precipitation of cellular components, and possibly even reducing the activity of certain membrane proteins. Figure 1: Schematic of the impacts…
Application Note 153: Differentiating Mono and Bivalent Antibody Binding in MCF7 Cell line
Understanding antibody binding and kinetics of therapeutic antibodies to tumor cells plays a critical role in advancing therapeutics. SPR is commonly used to study antibody binding reactions. However, it has…
Cell-based Measurement
…time. Have flexibility to meet your unique requirements. Benefit Process Cell-based assay with SPRm 200 Real-time, in-vitro, label-free kinetic measurements with simultaneous optical imaging for quantitative binding activity mapping on…
Technical Note 105: Introduction of SPR Microscopy
Surface Plasmon Resonance (SPR) is a widely used label-free detection technique for studying binding behavior of biomolecules. Since its commercialization in 1990’s [1], SPR has made vast advances in terms…
Application Note 145: KINETIC MEASUREMENTS OF COVID-19 VIRAL S1 PROTEIN WITH CELLULAR AND RECOMBINANT ACE2 RECEPTORS
…of the host cells is mainly through the viral attachment to angiotensin (Ang)-converting enzyme 2 (ACE2) present in the epithelial cells of the host1. During a viral infection, the trimeric…
BIOSENSING INSTRUMENT ATTENDING THE 2022 DISCOVERY ON TARGET CONFERENCE
…scientific solution that enables label-free direct measurement of binding affinity and kinetics of cell membrane proteins in their native environment along with a talk by Jonathan Brooks from Pfizer ”…
Application Note 144: Single Cell Apoptosis Monitoring with SPRm Impedance
…commonly used in material science for corrosion studies but its use in biological applications have grown rapidly and this technique is now used to monitor cell adhesion, growth, differentiation and…
Biosensing Instrument exhibiting at CHI’s 18th Annual PepTalk
…affinity and kinetics of cell membrane proteins in their native environment at the single cell level. Biosensing Instrument will be at booth #423. For more information about the conference: www.chi-peptalk.com…
Technologies
SURFACE PLASMON RESONANCE Surface Plasmon Resonance (SPR) has emerged as a powerful detection technique due to its high sensitivity and label-free capability. This optical-based real-time detection method delivers superior results…
Solutions
SPR Microscopy This award winning technology opens a new frontier in the label-free molecular interactions that combines SPR with optical microscopy. Designed specifically to measure binding affinity and kinetics of…
Technical Note 104: Concepts Behind EC-SPR
…= 5.85 x 1022 /cm2,em = -11.8 , and C = 20 m F/cm2, which leads to Dem ~ -0.002 per volt, corresponding to an angular shift of ~0.02° per…
Environment, Food & Beyond
…Detection of Biomolecules Application Note: 109 – Application of SPR to Bacteriology: Endotoxin/Protein Interaction Studies Application Note: 110 – Studying Protein Adsorption Properties with SPR Application Note: 111 – Measuring…
Electrochemical Measurements
…Determinations of Heavy Metal Ions Application Note: 111 – Measuring Surface Charge Density: A New Application with SPR Application Note: 113 – Flow-Through Electrochemical Surface Plasmon Resonance (SPR): Detection of…
Resources
We encourage you to explore our technical documentation below to learn more about Surface Plasmon Resonance Technology and how it can be applied to your research. APPLICATION NOTES These short…
Application Note 151: Studying Cell Binding Heterogeneity using SPRm 200
…to heterogenous HER2 molecules (Population A- non-glycosylated HER2 and Population B- Glycosylated HER2). The residues on HER2 comprising the primary amine groups are depicted as black dots, and the N-linked…