…was measured with the help from Biosensing Instruments researchers. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 133: Interaction…

…monolayers (SAMs)[2]. Such an electroactive organic thin film is a model system for probing interfacial electron transfer reactions [3], studies of molecular electronics, and construction of liquid crystal devices [4]….

…Gaussian distributions to extract the mean and 95% confidence interval of the cell populations. Lectin ka x 105 [1/M*s] kd x 10-4[1/s] KD [nM] SNA 1.27 8.47 7.03 WGA 0.43…

…Liu et al.[3], a new SPR biosensing configuration was used for real-time monitoring of VEGF secretion study. This novel design integrated a mini cell culture module into the flow cell…

…Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 126: GPCR Binding Assays with SPR Microscopy Download Now Note References Locatelli-Hoops, S et al,…

…death.(1) Despite the power of EIS, it lacks to provide spatial information which is essential to study the structure of the cells.  SPR microscopy (SPRm) Impedance is a technology that…

Surface Plasmon Resonance (SPR) is a powerful technique for measuring the binding kinetics of biomolecular interactions in a real-time and label-free manner [1].   In traditional SPR assays, the target…

…500 nM was achieved (FIG. 2), which is about 10 times lower than that reported by Fodey et al.[2] Moreover, the chip coated with amine-modified melamine can be regenerated and…

SPRm 200 system is the world’s first commercial Surface Plasmon Resonance Microscopy (SPRM) which combines the high spatial resolution of optical microscopy with the label-free sensing capability of SPR.   With…

…Dr. Scott Pollack from Sygnature Discovery for the samples, data and valuable insights. Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of…

…antibody association rate and stronger affinity16 HER2 and anti-HER2 FITC Interaction ka x 104 [1/M*s] kd x 10-4 [1/s] KD [nM] Native in-cell HER2, SKBR3 cells (glycosylated – peak) (non-glycosylated…

Membrane proteins are the main targets of therapeutic drugs and most of them carry some form of glycan structures, which play pivotal roles in protein folding, stability, cell communication, cell…

… Natural inhibitors of human α-thrombin such as heparin, hirudin [3], and hirugen [4] serve as anticoagulants in a variety of clinical applications. These examples show that the human α-thrombin is a…

…anti-BSA/BSA immune complex is relevant to studies of the receptor site of the red blood cells [1]. The five-channel BI-4500 SPR analysis system with integrated auto-sampler was used to study…

In recent years, SPR has been used as an alternative detector for monitoring elution of a variety of species (e.g., polysaccharides and proteins [1-4]) out of liquid chromatographic (LC) columns….

…| Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 127: Quantifying Antibody Binding to Membrane Proteins on Single Cells with SPR Microscopy Download Now Note References…

…measuring the signal attenuation at different positions. This work demonstrates that EC-SPR is a powerful tool for detecting and measuring reaction intermediates, and has potential to be used for studying…

…is label-free and simple to implement and does not need turbidity agents which are required for immunoturbidimetry. In this study, immunoglobulin (IgG) molecules in sera were accurately and rapidly quantified…

Surface Plasmon Resonance (SPR) has emerged as a sensitive, real-time and label free technique for quantifying biomolecular interactions.[1] By combining SPR with electrochemical methods, many new capabilities and applications are now…

SPR Microscopy (SPRM) is a powerful tool for measuring the binding kinetics of ligands or drug compounds with membrane proteins, including GPCRs. It has many other unique features that lead…

…is a label free technology utilized to study binding interactions of membrane proteins without extracting the proteins from the cell, ensuring intact native conformations of the membrane proteins.[3] Therefore, this technology…

…obtain using traditional labelled and label-free methods.  Measuring biophysical properties in the membrane target’s native environment in real time and label free provides insightful drug-receptor interactions information much earlier in…

…with increased acidity, Compound 13 is unlikely the appropriate compound to study GPR4 and its role in inflammatory bowel conditions. Compound 13 and its analogues’ pH dependence strongly limits their…

…negligible effects on binding due to biotinylation. Chemerin and CCRL2 Interaction ka x 105 [1/M*s] kd x 10-3[1/s] KD [nM] Unlabeled chemerin 3.11 1.16 5.49 Biotinylated chemerin 2.97 1.84 8.55…

SPRm 200 System is the world’s first commercial Surface Plasmon Resonance Microscopy (SPRM). It combines the high spatial resolution of optical microscopy with the powerful sensing capability of SPR, making…

…| Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 128: SPR Microscopy for Acid-Sensing Ion Channels Download Now Note References Lingueglia, E et…

…(ODN) and polynucleotide targets at sub-picomolar (sub-pM) [1]. Recently, low femtomolar level detections have even been achieved by utilizing ODN-capped gold nanoparticles [2] and enzyme-catalyzed reactions [4, 5]. This application…

Surface Plasmon Resonance (SPR) can be applied as a convenient, sensitive and label-free technique to study various surface phenomena. One such example is the Vroman effect exhibited by protein adsorption…

…biological processes and discovery of new drugs. Glycoproteins are membrane proteins that play an important role in cell recognition and communications. [3] Binding activities between lectins, a protein that bind…

…used as antiglaucoma agents to alleviate mountain sickness and to manage neurological disorders.[1] Acetazolamide (molecular weight = 222.22 Da) is one of the commonly used carbonic anhydrase inhibitors. The binding…

Understanding antibody binding and kinetics of therapeutic antibodies to tumor cells plays a critical role in advancing therapeutics. SPR is commonly used to study antibody binding reactions. However, it has…

Prussian blue is a pigment that is used to color paints, inks, textiles, and other commercial product (1). Prussian blue nanoparticles (PBNPs) are nanomaterials that present unique properties and excellent…

Membrane proteins play critical roles in cellular communications and are the most popular drug targets, accounting for over a half of the FDA approved drugs. One particularly important example of…

…detection of mutant and wild-type p53 in cancer cell lysates.[4] Table 1 Detection of p53 in normal and cancer cell lysates The standard deviations were computed from three replicate measurements Author:…

…potential inhibitors of β-site amyloid precursor protein cleaving enzyme 1 (BACE1). BACE1 is an enzyme that cleaves the transmembrane amyloid precursor protein to produce the amyloid beta (Aβ) peptides,[1] whose…

…(a cationic decapeptide) and determined the KD value to be about 157 nM [4]. Comparable ka and kd values were also reported [4]. Thus, endotoxin appears to bind to CD14…

Both electrochemical and surface plasmon resonance (SPR) techniques measure various processes taking place at or near an electrode surface. Combining the two techniques allows one to obtain new insight into…

…be with simple exponential functions. However, excellent fits were obtained by using a stretched exponential function, exp[-(t/t)b], with b~ 0.5. Stretched exponential processes are quite common in complex systems, such…

…Cu2+ [2]. Zhang et al. recently constructed a sensor surface covered with apo-metallothionein (i.e., metal-free metallothionein, a cysteine-rich protein) for selective determinations of Hg2+ and Cd2+ [3]. The principle behind…

…technology capable of differentiating antibody affinity and avidity on whole cells with emerging antibody modalities will be crucial in drug development. Traditional tools like ELISA study the effects of avidity…

…CK19 in spiked human plasma. Figure 3. Calibration curves for the detection of different concentrations of CK19 protein in PBS buffer and spiked 10% human plasma In conclusion, a carboxyl-GO…

…on neurons (3).  In this study, the binding interactions of two natural compounds with Αβ(1-42) were measured.  Ginnalin A (GA), a phenolic compound isolated from the red maple (Acer rubrum)…

SPR microscopy (SPRm) has emerged as a unique tool for measuring the affinity and kinetics of ligand binding to membrane proteins on the cells directly. The technology integrates the traditional…

…brain, the major components are peptides composed of 39–43 amino acid residues (referred to as the amyloid β or Aβ peptides).[1-2] One of the hypotheses for AD neuropathology is that…

…to remove immune complexes and apoptotic cells. Three different pathways comprise the complement system: the classical, lectin and alternative pathways [1]. While the complement system plays a central role in…

…microscope. These techniques are useful but also have their drawbacks. Shan et al [1] has recently reported a Surface Plasmon Resonance (SPR) method to measure surface charge density. The method,…

…the following equation [3] where Δθmax is the maximum SPR dip shift when the immobilized BSA molecules have all been bound to FA, and [FA] is the FA concentration. The…

…KD (C). Author: Nguyen Ly | Biosensing Instrument | Published May 4, 2020 DOWNLOAD PDF Download a PDF of Application Note 121: Protein Interactions in the Mycofactocin Biosynthetic Pathway Download…

…Figure 1: Schematic of anti Muc-4 antibody interactions with Muc-4 in glycosylated and partially deglycosylated pancreatic cancer cells. In this study, Surface Plasmon Resonance Microscopy (SPRM) was implemented to study…

Membrane proteins play a major role in cell biology and are an important class of receptors targeted by drug candidates being developed in the pharmaceutical industry.1,2  Techniques for studying the…

…values obtained are listed in Table 1. HER2 and anti-HER2 FITC Interaction ka x 104 [1/M*s] kd x 10-4[1/s] KD [nM] Native in-cell HER2, SKBR3 cells (glycosylated – peak) 4.16…

…epitope space of HER2 for potential clinical targets in a systematic manner. Surface plasmon resonance microscopy (SPRM) using BI’s SPRm200, integrates label-free, high-resolution SPR binding kinetic measurements with bright-field optical…

…relevant for drug development and testing. Surface plasmon resonance microscopy (SPRM), such as BI’s SPRm200, integrates label-free, high-resolution SPR binding kinetic measurements with bright-field optical microscopy for the study of…

…ligands compared to wild type I-domain.9 Surface Plasmon Resonance (SPR) was used to understand the affinity of the isolated mutants with C3d and to investigate the enhanced affinity of Q163C/Q309C…

Cell-based immunotherapy has gained great attention from researchers and pharmaceutical companies, particularly due to its promise to treat various cancers. Understanding the interaction between drug candidates and targets on the…

…glycoprotein on live cells in a cell medium. Glycoproteins are membrane proteins that play an important role in cell recognition and communications. (1,2) The binding of wheat germ agglutinin (WGA,…